Colorimetric and visual mercury(II) assay based on target-induced cyclic enzymatic amplification, thymine-Hg(II)-thymine interaction, and aggregation of gold nanoparticles.
本文报道了一种用于测定汞(II)的比色生物传感器和目视检测方法。该方法基于特异性胸腺嘧啶-Hg(II)-胸腺嘧啶(T-Hg2+-T)相互作用,该相互作用可诱导由外切酶III(Exo III)介导的循环酶促放大过程,并引起金纳米颗粒(AuNPs)聚集,使溶液颜色由红色变为紫色。在优化条件下,该比色检测在524 nm处表现最佳,检出限低...