Early and Sensitive Detection of Pathogens for Public Health and Biosafety: An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor.

传染病严重威胁公共卫生与全球生物安全。除空气传播外，病原微生物也可在污水等环境液体样本中检出。传统生化检测方法耗时长、成本效益低，且检测限限制早期诊断。本研究制备了直径125 μm的超细等离子体光纤探针，用于CRISPR/Cas12a介导的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测。暴露于光纤表面的单链DNA被Cas1...