Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method.
沃尔巴克氏体是约40%昆虫的内共生菌,但埃及伊蚊通常不含该菌。将 wAlbB 沃尔巴克氏体转入埃及伊蚊可使其丧失传播虫媒病毒的能力,因此释放感染蚊用于登革热等控制,并需现场监测感染状态。本文开发了基于 wsp 基因的环介导等温扩增(LAMP)方法,结合 DNA 链置换电化学生物传感器(BIOSENSOR)检测 wAlbB。LAMP ...