A superoxide anion biosensor based on direct electron transfer of superoxide dismutase on sodium alginate sol-gel film and its application to monitoring of living cells.

海藻酸钠（SA）溶胶-凝胶膜显著促进了超氧化物歧化酶（SOD）的直接电子转移，其形式电位为0.14 V，恰好位于O2•−/O2与O2•−/H2O2之间。SOD/SA修饰电极制备简单，无需任何中介体或促进剂。基于SOD/SA对O2•−的双分子识别和特异性反应，该SOD修饰电极可用于测定O2•−，具有低施加电位（0 V）、高选择性（无明...