Fluorescence enhancement of fluorescent unnatural streptavidin by binding of a biotin analogue with spacer tail and its application to biotin sensing.

本文报道了一种用于检测生物素的新型分子生物传感系统。作者采用四碱基密码子法，将荧光非天然氨基酸BODIPY-FL-氨基苯丙氨酸（BFLAF）位点特异性掺入链霉亲和素Trp120位点，制备Trp120BFLAF突变链霉亲和素。该突变蛋白保留足够的生物素结合活性。加入带双6-氨基己酸间隔尾的生物素类似物biotin-(AC5)2-hyd...