Electrochemiluminescence resonance energy transfer between graphene quantum dots and graphene oxide for sensitive protein kinase activity and inhibitor sensing.

本文首次提出一种基于石墨烯量子点(GQDs)为供体、氧化石墨烯(GO)为受体的电化学发光共振能量转移(ECL-RET)生物传感器，用于监测蛋白激酶活性。以酪蛋白激酶II(CK2)为模型，在CK2和ATP存在下，电极表面肽段被磷酸化；随后抗磷酸丝氨酸抗体偶联GO纳米复合体(Ab-GO)通过抗原-抗体作用捕获于磷酸化肽段/GQDs修饰电...