Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering.

本文报道了一种简便且超灵敏的酶生物燃料电池（EBFC）自供电生物传感策略，通过硫磷酸化介导的界面工程检测蛋白激酶A（PKA）活性及其抑制。该体系由GDH/CNT生物阳极和肽/AuNPs生物阴极组成。生物阴极上，肽CLRRASLG通过Au-S键固定于AuNPs/ITO电极，MCH封闭非特异性位点；在ATP-s存在下，PKA催化肽中丝氨...