Label-free fluorescent biosensor based on the target recycling and Thioflavin T-induced quadruplex formation for short DNA species of c-erbB-2 detection.

乳腺癌的非侵入性早期诊断是提高生存率和保留乳房机会的关键。本文开发了一种基于核酸外切酶III辅助靶标循环和硫黄素T诱导G-四链体形成的无标记荧光生物传感器，用于唾液中c-erbB-2短DNA片段的检测。该体系由G/P双链DNA探针、ThT和Exo III组成。靶标T1与探针杂交后形成平末端，Exo III逐步消化探针并释放T1和富含...