Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants.

ALG-2是五EF手蛋白家族原型成员，通过Alix C端富含脯氨酸区（含四个串联PXY重复）结合。人磷脂翻转酶3（PLSCR3）N端有类似序列ABS-1。本研究用共免疫沉淀、GST-ALG-2下拉和生物素标记ALG-2印迹发现，ALG-2以钙依赖方式与HEK293细胞中PLSCR3相互作用。ALG-2可变剪接异构体GST-ALG-2...