1 条结果 关键词:空心芯光纤 ×

Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume.

Journal of biomedical optics 2010 Padmanabhan S, Shinoj VK, Murukeshan VM, Padmanabhan P

本文报道了一种基于空心芯光子晶体光纤(HC-PCF)的简单光学蛋白检测方法。研究将MCF-7乳腺癌细胞裂解液中的雌激素受体α(ERα)固定于HC-PCF空心芯内,采用抗ERα一抗与Alexa Fluor 488(绿色荧光)或Alexa Fluor 555(红色荧光)标记的山羊抗兔IgG二抗进行免疫识别。通过荧光显微镜观察ERα的荧光...