Non-invasive diagnosis of bladder cancer by detecting telomerase activity in human urine using hybridization chain reaction and dynamic light scattering.

膀胱癌检测的金标准膀胱镜和组织学检查主观性强、费用高且有创。本文开发了一种通过检测人尿中端粒酶活性来无创诊断膀胱癌的方法。端粒酶底物TS引物在端粒酶存在下被延伸为(TTAGGG)n重复序列；延伸产物作为引发剂触发两个发夹探针H1和H2的杂交链反应，形成带缺口双链DNA。HCR产物通过尾部序列与DNA修饰金纳米颗粒探针杂交，引起AuN...