A SERS Biosensor Based on Functionalized Au-SiNCA Integrated with a Dual Signal Amplification Strategy for Sensitive Detection of Telomerase Activity During EMT in Laryngeal Carcinoma.

本研究构建了一种基于功能化金-硅纳米锥阵列（Au-SiNCA）的表面增强拉曼散射（SERS）生物传感器，并整合链置换放大（SDA）与催化发夹自组装（CHA）双信号放大策略，用于检测喉癌上皮间质转化（EMT）过程中的端粒酶活性。首先通过胶体球模板辅助反应离子刻蚀和磁控溅射制备有序 Au-SiNCA，并用发夹 DNA2（H2）修饰形成捕...