Application of tetrahedral -deoxyribonucleic acid electrochemistry platform coupling aptazymes and hybridized hairpin reactions for the measurement of extracellular adenosine triphosphate in plants.
细胞外三磷酸腺苷(eATP)是重要的生物信号转导分子。由于eATP浓度极低且存在时空分布,准确检测仍具挑战。本文提出基于四面体DNA(T-DNA)修饰电极,并结合同源杂交链反应(HCR)与G-四联体/血红素(G4/Hemin)DNAzyme双信号放大的eATP检测方法。ATP适配体与RNA切割DNAzyme组成分裂型适配酶(apta...