2 条结果 关键词:胰蛋白酶 ×

Remote biosensor for the determination of trypsin by using nanoporous anodic alumina as a three-dimensional nanostructured material.

Scientific reports 2020 Amouzadeh Tabrizi M, Ferré-Borrull J, Marsal LF

胰蛋白酶在人体真实样品中的测定是评估胰腺疾病的常规医学检查。本文报道了一种基于干涉反射光谱(IRS)的胰蛋白酶生物传感器。为此,将脲酶和荧光素5(6)-异硫氰酸酯(FLITC)固定在纳米孔阳极氧化铝(NAA)上。该传感器的工作原理基于脲酶与尿素反应引起溶液pH变化,从而改变FLITC在胰蛋白酶存在下的吸光能力。脲酶催化尿素水解产生氨...

Highly sensitive IRS based biosensor for the determination of cytochrome c as a cancer marker by using nanoporous anodic alumina modified with trypsin.

Biosensors & bioelectronics 2020 Amouzadeh Tabrizi M, Ferré-Borrull J, Marsal LF

检测人血清中细胞色素c(Cyt C)是评估癌症的常规医学检测。本文构建了基于干涉反射光谱(IRS)的生物传感器用于检测 Cyt C。首先制备纳米孔阳极氧化铝(NAA),随后用3-氨基丙基三甲氧基硅烷对 NAA 孔壁进行功能化得到 NAA-NH2,再将胰蛋白酶(Tryp)固定于 NAA 孔壁。该 IRS 传感器检测 Cyt C 的原理...