Development of multiplex loop mediated isothermal amplification (m-LAMP) label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira.

本文报道了一种基于标记的多重环介导等温扩增（m-LAMP）金纳米颗粒侧流层析试纸条生物传感器，用于快速、简便地检测致病钩端螺旋体（pathogenic Leptospira）DNA。该试纸条可同时检测目标DNA模板、LAMP内参扩增产物和层析质控信号，采用标记识别体系，无需额外探针杂交和孵育步骤。试纸条上设置三条线：检测线捕获致病钩...