Excited state structural events of a dual-emission fluorescent protein biosensor for Ca²⁺ imaging studied by femtosecond stimulated Raman spectroscopy.
荧光蛋白(FPs)是一类受光激发后发出特征颜色荧光的发光生物分子。钙调蛋白(CaM)-绿色荧光蛋白(GFP)嵌合体已被用于光学检测钙离子(Ca2+)。本研究采用飞秒受激拉曼光谱(FSRS)考察一种遗传编码钙离子光学成像生物传感器 GEM-GECO1;该传感器在无 Ca2+ 时发绿光,结合 Ca2+ 后发蓝光。时间分辨 FSRS 数据...