Production of a broad specificity antibody for the development and validation of an optical SPR screening method for free and intracellular microcystins and nodularin in cyanobacteria cultures.
本研究制备并表征了一种高灵敏度、广谱特异性单克隆抗体,用于微囊藻毒素检测,并建立快速表面等离子共振(SPR)光学生物传感器免疫分析方法。该抗体交叉反应为:MC-LR 100%、MC-RR 108%、MC-YR 68%、MC-LA 69%、MC-LW 71%、MC-LF 68%、节球藻毒素 94%。将 MC-LR 共价固定于 CM5 ...