2 条结果 关键词:谷胱甘肽过氧化物酶 ×

An effective electrochemical biosensing platform for the detection of reduced glutathione.

Artificial cells, nanomedicine, and biotechnology 2016 Anik Ü, Çubukçu M, Ertaş FN

本文报道了一种用于检测还原型谷胱甘肽(GSH)的电化学生物传感器。该传感器以玻璃碳糊电极(GCPE)为基底,先通过循环伏安法将铂纳米颗粒(Pt-NP)电沉积于电极表面,再采用明胶固定谷胱甘肽过氧化物酶(GSH-Px),并用戊二醛交联成膜。检测基于在过氧化氢(H2O2)存在下 GSH 被氧化为其二硫键形式,利用差分脉冲伏安法(DPV)...

Selection of phage antibodies with GPX activity by combination of phage displayed antibody library with chemical modification and their characterization using a surface plasmon resonance biosensor.

Talanta 2005 Mu Y, Song D, Li Y, Zhang HQ 等 7 人

谷胱甘肽过氧化物酶(GPX)是清除活性氧的重要抗氧化酶。为获得人源化GPX催化抗体,本研究以噬菌体展示人抗体库为材料,经多轮筛选获得新型抗体。以三种S-2,4-二硝基苯基谷胱甘肽酯半抗原GSH-S-DNP-Bu(B)、GSH-S-DNP-He(H)和GSH-S-DNP-cHe(C)为靶标,经ELISA四轮筛选,得到具有谷胱甘肽(GS...