Enhanced functional DNA biosensor for distance-based read-by-eye quantification of various analytes based on starch-hydrolysis-adjusted wettability change in paper devices.

本文报道了一种低成本、无需仪器、可肉眼定量检测多种分析物的功能DNA生物传感器。该传感器将分析物导向的酶包被微珠探针组装与基于淀粉水解调节纸张润湿性的纸基微流控距离测量读出相结合，实现稳健而高效的信号放大。超顺磁微珠共价修饰末端生物素化DNA链，用于锚定分析物特异性适配体或脱氧核酶；识别分析物后，暴露的生物素通过生物素-链霉亲和素作...