Isolation and Purification of Organophosphorus Hydrolases Secreted from Acetone-acclimated Phosphorus Accumulating Organisms and Study of Their Properties for Hydrophobic Organophosphorus Sensor.

本研究针对高疏水性有机磷（OP）农药检测难题，采用丙酮对聚磷菌（PAOs）进行驯化，以开发基于微生物来源酶的疏水有机磷生物传感器。通过三步培养与驯化，在多种 PAOs 中仅杆状细菌存活。将驯化 PAOs 释放的胞外酶经硫酸铵盐析、透析膜处理及 DEAE-Sepharose FF 阴离子交换柱纯化，成功分离出两种酶组分。两种酶均对对硝...