A novel amperometric alcohol biosensor developed in a 3rd generation bioelectrode platform using peroxidase coupled ferrocene activated alcohol oxidase as biorecognition system.

本研究通过微波辅助部分展开技术将激活剂二茂铁（ferrocene, Fc）物理包埋于醇氧化酶（alcohol oxidase, AOx）蛋白基质中，制备了二茂铁包埋醇氧化酶（FcAOx），使酶催化效率（Kcat/Km）提高约两倍。高级分析技术表明，Fc以约3:1的摩尔比通过静电作用稳定结合于参与酶功能的色氨酸（Trp）残基。传感器采...