Electrochemical biosensor based on enzyme substrate as a linker: Application for aldolase activity with pectin-thionine complex as recognization element and signal amplification probe.
报道了一种基于酶底物连接策略构建电化学生物传感器的新方法,并建立了以果胶-硫堇复合物(PTC)作为识别元件和信号探针的电化学方法检测醛缩酶活性。醛缩酶底物1,6-二磷酸果糖(FBP)可通过与磁性纳米颗粒(MNPs)/氨基苯硼酸(APBA)的强结合,以及与电极表面对苯二胺(PDA)反应形成磷酸酰胺键,实现连接作用。醛缩酶可逆催化底物转...