A single electrochemical biosensor for detecting the activity and inhibition of both protein kinase and alkaline phosphatase based on phosphate ions induced deposition of redox precipitates.
蛋白激酶A(PKA)和碱性磷酸酶(ALP)是多种疾病中具有重要临床意义的酶。本文开发了一种简单、通用的电化学生物传感器,用于同时检测PKA和ALP的活性及其抑制情况。PKA可水解三磷酸腺苷(ATP),ALP可水解焦磷酸盐(PPi),两种反应均产生磷酸离子(Pi),且Pi生成量与酶活性成正比。该传感器基于Pi与钼酸盐反应在电极表面形成...