An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework.

本文首次开发一种基于铁金属有机框架（FeMOF）自牺牲标签生成普鲁士蓝增强准可逆氧化还原信号的电化学生物传感器，用于T4多核苷酸激酶（PNK）的信号开启检测。3'-巯基端修饰FeMOF@AuNPs的发夹DNA探针作为PNK底物。PNK存在使探针5'端磷酸化，随后作为λ外切酶底物；λ外切酶从茎部切除5'单核苷酸，打开发夹结构并释放单链...