An enzyme-free and label-free assay for copper(II) ion detection based on self-assembled DNA concatamers and Sybr Green I.
本文报道了一种无酶、无标记的荧光开启型生物传感器,用于铜(II)离子(Cu2+)的放大检测。该体系以Cu2+特异性DNAzyme为识别元件,在Cu2+存在下不可逆切割底物链,释放的目标DNA片段作为催化剂,触发两条辅助探针SP1和SP2在羧基化磁珠表面级联自组装,形成长双链DNA串联体。随后加入Sybr Green I(SG),其与...