Ultrasensitive electrochemical biosensor for detection of DNA from Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification.

本文设计了一种简单、超灵敏且特异性的电化学生物传感器，通过耦合目标诱导链置换与切口内切酶信号放大，用于检测枯草芽孢杆菌（Bacillus subtilis）的特定DNA序列。目标DNA（TD）选自枯草芽孢杆菌16S rDNA高变区，采用差分脉冲伏安法（DPV）检测，线性范围为0.1 fM至20 fM，检出限为0.08 fM（3s空白...