Label-free electrochemical detection of short sequences related to the hepatitis B virus using 4,4'-diaminoazobenzene based on multiwalled carbon nanotube-modified GCE.
报道了一种基于4,4'-二氨基偶氮苯(4,4'-DAAB)和多壁碳纳米管(MWNT)修饰玻碳电极(GCE)的新型无标记电化学DNA生物传感器,用于检测与乙型肝炎病毒(HBV)相关的短DNA序列杂交。采用差分脉冲伏安法(DPV)监测杂交事件,当固定化探针与互补目标DNA杂交后,4,4'-DAAB的还原峰电流明显下降。对照实验表明,非互...