Identification and characterization of 4-hexylbenzoic acid and 4-nonyloxybenzoic acid as substrates of CYP102A1.

CYP102A1是高效中长链脂肪酸羟化酶，可接受广泛非天然底物。通过BD Oxygen Biosensor System筛选，鉴定4-己基苯甲酸（HBA）和4-壬氧基苯甲酸（NOBA）为CYP102A1底物。光谱结合研究显示二者结合于活性位点，Kd分别为2.6±0.1 μM和1.9±0.2 μM。存在HBA和NOBA时NADPH消耗...