A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs.

AMP-激活蛋白激酶（AMPK）是细胞能量代谢的核心调控激酶，也是2型糖尿病等代谢疾病的潜在治疗靶点。尽管体外研究揭示了AMPK受AMP/ATP、LKB1和CaMKK2等复杂调控，其在活体细胞与器官中的异质性仍不清楚。作者将长柔性EV连接肽引入AMPK FRET传感器AMPKAR，并将YFP替换为FRET倾向更强的YPet，构建了高...