Repurposing the Sb(III)-specific efflux and sequestration system (ant operon) to mitigate antimonite cross-talk in ArsR-based bacterial arsenite sensors.

基于ArsR的全细胞生物传感器可灵敏比色检测亚砷酸盐[As(III)]，但对亚锑酸盐[Sb(III)]的宽泛响应限制现场特异性。Comamonas testosteroni JL40的ant操纵子通过外排ATP酶AntA、金属伴侣蛋白AntC和调控蛋白AntR赋予Sb(III)选择性抗性。作者将antA、antC及三个antR同源基...