Method for measurement of bacillithiol redox potential changes using the Brx-roGFP2 redox biosensor in
金黄色葡萄球菌在感染过程中常遭遇活性氧,依赖低分子量硫醇 bacillithiol(BSH)维持胞内氧化还原稳态。遗传编码的氧化还原敏感绿色荧光蛋白 roGFP2 可实时监测氧化还原电位,但与谷胱甘肽体系特异性有限。作者将金黄色葡萄球菌 bacilliredoxin(Brx)与 roGFP2 融合,构建 Brx-roGFP2 生物传...