2 条结果 关键词:Hoechst染料 ×

Visual fluorescence detection of H2O2 and glucose based on "molecular beacon"-hosted Hoechst dyes.

The Analyst 2015 Lu LF, Li YY, Zhang M, Shi G

本文设计了一种无标记分子信标(MB)类荧光生物传感器,用于检测过氧化氢(H2O2)和葡萄糖。该体系基于AT富集单链DNA(ATprobe)对Hoechst染料的荧光调控,其中Hg2+和半胱氨酸(Cys)作为激活/调节元件。ATprobe可在Hg2+诱导下通过T–Hg2+–T复合物形成发夹结构,为Hoechst染料提供结合宿主,使其荧...

"Molecular beacon"-directed fluorescence of Hoechst dyes for visual detection of Hg(II) and biothiols and its application for a logic gate.

Chemical communications (Cambridge, England) 2013 Zhang M, Le HN, Jiang XQ, Ye BC

本研究开发了一种分子信标样DNA发夹生物传感器,利用Hoechst染料对富AT双链DNA小沟结合后的荧光增强效应,实现Hg2+和生物硫醇的无标记荧光检测。ATprobe在Hg2+诱导下形成T-Hg2+-T茎环发夹,为Hoechst染料提供结合位点,使其荧光由弱变强;随后Cys、Hcys、GSH等生物硫醇通过硫醇-Hg2+作用夺取Hg...