Determination of collagen type IV by Surface Plasmon Resonance Imaging using a specific biosensor.

血清IV型胶原（COLIV）是潜在肿瘤标志物，结直肠癌、胃癌、肺癌、肝癌及乳腺癌患者血清中浓度升高。本研究开发了一种用于表面等离子共振成像（SPRI）技术的COLIV检测生物传感器。传感器以镀金玻璃芯片为基底，通过半胱胺连接层固定小鼠抗人IV型胶原单克隆抗体。该传感器在10–300 ng/mL范围内选择性响应，检出限为2.4 ng/...