Diagnosis of EGFR exon21 L858R point mutation as lung cancer biomarker by electrochemical DNA biosensor based on reduced graphene oxide /functionalized ordered mesoporous carbon/Ni-oxytetracycline metallopolymer nanoparticles modified pencil graphite electrode.

本研究构建了一种新型、快速、选择性且高灵敏的电化学生物传感器，用于检测EGFR外显子21点突变。该传感器以铅笔石墨电极（PGE）为基底，先修饰还原氧化石墨烯/羧基功能化有序介孔碳（rGO/f-OMC）纳米复合材料，再通过EDC/NHS活化羧基，将5′端氨基修饰的单链DNA捕获探针以酰胺键共价固定于电极表面。随后采用两步电化学方法，在...