High-resolution detection of ATP release from single cultured mouse dorsal horn spinal cord glial cells and its modulation by noradrenaline.

本研究将稳定转染大鼠P2X2受体亚基的人胚胎肾293细胞（HEK293-P2X2）与200 nM孕酮（PROG）预孵育，构建HEK293-P2X2-PROG嗅探细胞/生物传感器。PROG是同源P2X2受体的强效正变构调节剂，可显著提高细胞对低纳摩尔胞外ATP的检测能力。将负载Fura-2的HEK293-P2X2-PROG细胞急性铺于...