High-yield production and functional analysis of novel rhizobial-type glutaminase-free L-asparaginase from Paenibacillus thiaminolyticus.

L-天冬酰胺酶因可用于急性淋巴细胞白血病治疗、食品工业及生物传感器设计而受到关注。本文从 Paenibacillus thiaminolyticus 中克隆并重组表达了一种属于根瘤菌型（Rhizobium etli 型）L-天冬酰胺酶同工酶 1（ASN339Pt iso 1），该类别研究较少。在 E. coli Lemo21(DE3...