The assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ions.
本研究从Puntius javanicus肝组织中提取粗胆碱酯酶(ChE),并用procainamide-sepharyl 6B亲和层析进行纯化。以S-丁酰硫代胆碱碘化物(BTC)为特异性合成底物,其最大反应速度(Vmax)和米氏常数(Km)分别为53.49 μmole/min/mg和0.23 mM,催化效率Vmax/Km为0.23...