A strategy of screening and binding analysis of bioactive components from traditional Chinese medicine based on surface plasmon resonance biosensor.
阐明中药活性成分对理解中药作用机制、促进合理用药及中药新药开发具有重要意义。表面等离子共振(SPR)技术在该领域具有应用前景。本研究提出一种基于SPR的整合策略,用于筛选和分析中药主要活性成分,并以白芍(Radix Paeoniae Alba, RPA)为例,通过肿瘤坏死因子受体1(TNF-R1)解析其抗炎机制。首先,利用SPR筛选...