Structure of a NADPH-dependent blue fluorescent protein revealed the unique role of Gly176 on the fluorescence enhancement.

本文报道了来自副溶血弧菌CKM-1的NADPH依赖蓝色荧光蛋白BFPvvD8的结构与荧光增强机制。BFPvv在紫外光激发下发射蓝色荧光，此前通过定向进化获得的BFPvvD7突变体荧光强度提高约4倍。作者进一步在D7基础上引入N58Y、A62T和K199R三个突变，获得荧光更强的BFPvvD8。为阐明增强机制，解析了BFPvvD8与N...