Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy.
本研究提出一种无标记荧光生物传感器,用于超灵敏、简便地检测金黄色葡萄球菌(Staphylococcus aureus)的mecA基因。该传感器采用外切酶III(Exo III)辅助的级联信号放大策略,以3′端突出发夹探针(HP)作为目标识别元件,以笼式G-四链体作为信号报告元件。无mecA基因时,HP探针不能被Exo III消化,G...