Highly sensitive and specific electrochemical biosensor for microRNA-21 detection by coupling catalytic hairpin assembly with rolling circle amplification.

微小RNA（miRNA）在基因调控中发挥重要作用，是潜在生物标志物；因其尺寸小、易降解、序列同源且表达水平低，检测具有挑战。本文以miRNA 21为对象，构建结合催化发夹组装（CHA）与滚环扩增（RCA）双信号放大策略的电化学生物传感器。金电极表面自组装巯基化捕获探针，并用MCH、鲑鱼精子DNA和BSA封闭。目标miR-21催化生物...