Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-L-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus.

本文构建了羧基功能化氧化石墨烯（GO-COOH）与电聚合聚-L-赖氨酸（PLLy）修饰的玻碳电极（GCE），用于制备电化学脱氧核糖核酸（DNA）生物传感器。氨基修饰的探针单链DNA（NH2-ssDNA）通过酰胺键共价固定于GO-COOH/PLLy/GCE表面，固定稳定并可进一步与目标单链DNA杂交。采用差分脉冲伏安法（DPV）监测杂...