High-Fidelity CRISPR/Cas13a
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) (CRISPR/Cas) system innovates a next-generation biosensor due to its...
Spectroscopic Analysis of Fe Ion-Induced Fluorescence Quenching of the Green Fluorescent Protein ZsGreen.
The fluorescence of fluorescent proteins (FPs) is quenched when they are exposed to certain transition metals, which makes them promising receptor materials for metal ...
Divalent Cation Dependence Enhances Dopamine Aptamer Biosensing.
Oligonucleotide receptors (aptamers), which change conformation upon target recognition, enable electronic biosensing under high ionic-strength conditions when coupled...
A CRISPR-Cas12a-derived biosensor enabling portable personal glucose meter readout for quantitative detection of SARS-CoV-2.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly throughout the whole world and caused significant difficulties in the prevention and co...
Selective and Sensitive ZnO Quantum Dots Based Fluorescent Biosensor for Detection of Cysteine.
In the present article, a novel and effective ZnO quantum dots-based fluorescent probe has been developed for the detection of cysteine in different solutions. Firstly...
Analysis of bioavailable toluene by using recombinant luminescent bacterial biosensors with different promoters.
In this study, we constructed recombinant luminescent Escherichia coli with T7, T3, and SP6 promoters inserted between tol and lux genes as toluene biosensors and eval...
A photoelectrochemical enzyme biosensor based on functionalized hematite microcubes for rutin determination by square-wave voltammetry.
A photoelectrochemical biosensing strategy for the highly sensitive detection of the flavonoid rutin was developed by synergizing the photoelectrocatalytic properties ...
Gold nanocluster-europium(III) ratiometric fluorescence assay for dipicolinic acid.
A ratiometric fluorescence assay was designed for determination of dipicolinic acid (DPA), a spore-specific compound which is used as a biomarker for Bacillus anthraci...
Recent development of antibiotic detection in food and environment: the combination of sensors and nanomaterials.
In recent years, the abuse of antibiotics has led to the pollution of soil and water environment, not only poultry husbandry and food manufacturing will be influenced ...
Enhancing the Sensitivity of Photoelectrochemical DNA Biosensing Using Plasmonic DNA Barcodes and Differential Signal Readout.
Photoelectrochemical biosensors hold great promise for sensitive bioanalysis; however, similar to their electrochemical analogues, they are highly affected by the vari...
BIO bragg gratings on microfibers for label-free biosensing.
Discovering nanoscale phenomena to sense biorecognition events introduces new perspectives to exploit nanoscience and nanotechnology for bioanalytical purposes. Here w...
Entropy-driven electrochemiluminescence ultra-sensitive detection strategy of NF-κB p50 as the regulator of cytokine storm.
2019 novel coronavirus (2019-nCoV) with strong contagion in the crowd, has ravaged worldwide and severely impacts the human health and epidemic prevention system, by p...
Spatiotemporal Characterization of GPCR Activity and Function during Endosomal Trafficking Pathway.
G protein-coupled receptor (GPCR) is activated by extracellular signals. After their function at plasma membrane, GPCRs are internalized to be desensitized, while emer...
Discovery of an Allosteric Ligand Binding Site in SMYD3 Lysine Methyltransferase.
SMYD3 is a multifunctional epigenetic enzyme with lysine methyltransferase activity and various interaction partners. It is implicated in the pathophysiology of cancer...
Efficient Curing Sacrificial Agent-Induced Dual-Heterojunction Photoelectrochemical System for Highly Sensitive Immunoassay.
A photoelectrochemical (PEC) biosensor is a very efficient and sensitive detection technology for the quick and effective conversion of light to electrical signals. Ho...
Label-Free Electrochemical Immunosensor for Ultrasensitive Detection of Carbohydrate Antigen 125 Based on Antibody-Immobilized Biocompatible MOF-808/CNT.
In this work, a nanocomposite of Zr-trimesic acid MOF (MOF-808) with carbon nanotube (CNT) was synthesized through an in situ formation of MOF-808 on the activated CNT...
Rational design of direct electron transfer type l-lactate dehydrogenase for the development of multiplexed biosensor.
Facile biosensor-based system for on-site quantification of total viable counts in food and environmental swabs.
We describe a new biosensor platform for rapid and simple quantification of total aerobic viable counts of bacteria (TVC) in food and environmental swabs by oxygen res...
Effects of Experimental Conditions on the Signaling Fidelity of Impedance-Based Nucleic Acid Sensors.
Electrochemical impedance spectroscopy (EIS), an extremely sensitive analytical technique, is a widely used signal transduction method for the electrochemical detectio...
Photo-driven self-powered biosensors for ultrasensitive microRNA detection based on metal-organic framework-controlled release behavior.
We developed a "signal-on" self-powered biosensing strategy by taking full advantage of both photoelectrochemical biofuel cells (PBFCs) and metal-organic framework (MO...