共 1342 条结果
Applied microbiology and biotechnology
2015
Fan L, Zhao M, Wang Y
The CotA laccase from Bacillus subtilis WD23 was successfully overexpressed in Pichia pastoris, and the production level reached 891.2 U/L. The recombinant CotA laccas...
PloS one
2015
Litzlbauer J, Schifferer M, Ng D, Fabritius A 等 6 人
Biosensors based on Förster Resonance Energy Transfer (FRET) between fluorescent protein mutants have started to revolutionize physiology and biochemistry. However, ma...
Protein science : a publication of the Protein Society
2015
Whitfield JH, Zhang WH, Herde MK, Clifton BE 等 8 人
Biosensors for signaling molecules allow the study of physiological processes by bringing together the fields of protein engineering, fluorescence imaging, and cell bi...
Scientific reports
2015
Chen W, Yan Y, Zhang Y, Zhang X 等 6 人
A simple and versatile colorimetric biosensor has been developed for sensitive and specific detection of a wide range of biomolecules, such as oligonucleotides and apt...
Science signaling
2015
Mills E, Petersen E, Kulasekara BR, Miller SI
Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that transduces internal and external signals and regulates bacterial motility and biofilm formation. Some org...
Methods in molecular biology (Clifton, N.J.)
2015
Wang H, Lang Q, Liang B, Liu A
The conventional enzyme-based biosensor requires chemical or physical immobilization of purified enzymes on electrode surface, which often results in loss of enzyme ac...
Nanoscale research letters
2015
Zhang J, Irannejad M, Yavuz M, Cui B
Nanofabrication technology plays an important role in the performance of surface plasmonic devices such as extraordinary optical transmission (EOT) sensor. In this wor...
Biosensors & bioelectronics
2015
Pal MK, Rashid M, Bisht M
A multiplexed MNPs-Abs based fluorescence spectroscopic system in analysis of serum biomarkers; CA-125, β2-M and ApoA1 for the early detection of ovarian cancer was fi...
Biosensors & bioelectronics
2015
Kumar S, Kumar S, Srivastava S, Yadav BK 等 8 人
UNLABELLED: We report results of the studies relating to the fabrication of a paper based sensor comprising of poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate)...
Journal of biomedical optics
2015
Peter B, Nador J, Juhasz K, Dobos A 等 8 人
The in situ observation of cell movements and morphological parameters over longer periods of time under physiological conditions is critical in basic cell research an...
Current opinion in chemical biology
2015
Zhang J, Jensen MK, Keasling JD
In a sustainable bioeconomy, many commodities and high value chemicals, including pharmaceuticals, will be manufactured using microbial cell factories from renewable f...
Nature cell biology
2015
Case LB, Baird MA, Shtengel G, Campbell SL 等 7 人
Focal adhesions (FAs) link the extracellular matrix to the actin cytoskeleton to mediate cell adhesion, migration, mechanosensing and signalling. FAs have conserved na...
Scientific reports
2015
Zhang W, Li X, Zou R, Wu H 等 7 人
Novel water-dispersible and biocompatible chitosan-functionalized graphene (CG) has been prepared by a one-step ball milling of carboxylic chitosan and graphite. Prese...
The Analyst
2015
Zhang Z, Hao J, Xiao T, Yu P 等 5 人
This study demonstrates a new strategy to develop online electrochemical systems (OECSs) for continuously monitoring neurochemicals by efficiently integrating in vivo ...
Analytical biochemistry
2015
Abdolrahim M, Rabiee M, Alhosseini SN, Tahriri M 等 6 人
Acute myocardial infarction (AMI) is the leading cause of death among cardiovascular diseases. Among the numerous attempts to develop coronary marker concepts into cli...
Analytical biochemistry
2015
Branchini BR, Southworth TL, Fontaine DM, Kohrt D 等 9 人
Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been adapted for a variety of applications, including gene reporter assa...
Talanta
2015
Li R, Feng F, Chen ZZ, Bai YF 等 7 人
A new method for real-time detection of carcinoembryonic antigen (CEA) in human serum with high sensitivity and selectivity using surface plasmon resonance (SPR) biose...
Talanta
2015
Rahman MM, Asiri AM
One-step approach of cholesterol biosensor was fabricated onto smart micro-chips based on cholesterol oxidase (ChOx) co-immobilized thioglycolic acid self-assembled mo...
Talanta
2015
Cennamo N, Pesavento M, Lunelli L, Vanzetti L 等 7 人
The introduction of new compact systems for sensitive, fast and simplified analysis is currently playing a substantial role in the development of point-of-care solutio...
Langmuir : the ACS journal of surfaces and colloids
2015
Bertok T, Šedivá A, Filip J, Ilcikova M 等 14 人
Impedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human ser...