Live-Cell STED Microscopy with Genetically Encoded Biosensor.
Of the various super-resolution techniques, stimulated emission depletion (STED) microscopy achieves the best temporal resolution at high spatial resolution, enabling ...
"Signal-on" photoelectrochemical biosensor for sensitive detection of human T-Cell lymphotropic virus type II DNA: dual signal amplification strategy integrating enzymatic amplification with terminal deoxynucleotidyl transferase-mediated extension.
A novel "signal-on" photoelectrochemical (PEC) biosensor for sensitive detection of human T-cell lymphotropic virus type II (HTLV-II) DNA was developed on the basis of...
A method for selecting structure-switching aptamers applied to a colorimetric gold nanoparticle assay.
Small molecules provide rich targets for biosensing applications due to their physiological implications as biomarkers of various aspects of human health and performan...
Enzyme-based microfluidic chip coupled to graphene electrodes for the detection of D-amino acid enantiomer-biomarkers.
An electrochemical microfluidic strategy for the separation and enantiomeric detection of D-methionine (D-Met) and D-leucine (D-Leu) is presented. These D-amino acids ...
Environmental pollutants directly affect the liver X receptor alpha activity: Kinetic and thermodynamic characterization of binding.
Liver X receptor is a ligand-activated transcription factor, which is mainly involved in cholesterol homeostasis, bile acid and triglycerides metabolism, and, as recen...
Tricyclic Antidepressant Amitriptyline-induced Glial Cell Line-derived Neurotrophic Factor Production Involves Pertussis Toxin-sensitive Gαi/o Activation in Astroglial Cells.
Further elaborating the mechanism of antidepressants, beyond modulation of monoaminergic neurotransmission, this study sought to elucidate the mechanism of amitriptyli...
A rationally designed peptidomimetic biosensor for sialic acid on cell surfaces.
We have developed peptidomimetic sialic acid (Sia) biosensors using boronic acid and arginine groups on the peptide backbone. The designed peptides were conjugated to ...
Visual fluorescence detection of H2O2 and glucose based on "molecular beacon"-hosted Hoechst dyes.
In this work, a label-free molecular beacon (MB)-like biosensor is designed for the determination of H2O2 and glucose based on the fluorescence regulation of Hoechst d...
Detection of human leukocyte antigen biomarkers in breast cancer utilizing label-free biosensor technology.
According to the American Cancer Society, more than 200,000 women will be diagnosed with invasive breast cancer each year and approximately 40,000 will die from the di...
Impedimetric DNA-biosensor for the study of dopamine induces DNA damage and investigation of inhibitory and repair effects of some antioxidants.
A simple and inexpensive methodology was used to develop a new method in order to inspect the DNA damage due to dopamine and some ionic metals. In addition, the inhibi...
Computational selection of RNA aptamer against angiopoietin-2 and experimental evaluation.
Angiogenesis plays a decisive role in the growth and spread of cancer and angiopoietin-2 (Ang2) is in the spotlight of studies for its unique role in modulating angiog...
An enzyme-free and label-free fluorescent biosensor for small molecules by G-quadruplex based hybridization chain reaction.
An enzyme-free and label-free fluorescent biosensor is developed by G-quadruplex-based hybridization chain reaction (HCR) for small molecules, using adenosine triphosp...
A glucose oxidase-coupled DNAzyme sensor for glucose detection in tears and saliva.
Biosensors have been widely investigated and utilized in a variety of fields ranging from environmental monitoring to clinical diagnostics. Glucose biosensors have tri...
A novel and simple cell-based electrochemical impedance biosensor for evaluating the combined toxicity of DON and ZEN.
In this study, a novel and simple cell-based electrochemical biosensor was developed to assess the individual and combined toxicity of deoxynivalenol (DON) and zearale...
Gq-DREADD Selectively Initiates Glial Glutamate Release and Inhibits Cue-induced Cocaine Seeking.
BACKGROUND: Glial cells of the central nervous system directly influence neuronal activity by releasing neuroactive small molecules, including glutamate. Long-lasting ...
High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions.
This chapter describes the implementation of the p53-hDM2 protein-protein interaction (PPI) biosensor (PPIB) HCS assay to identify disruptors of p53-hDM2 PPIs. Recombi...
Resonant waveguide grating for monitoring biomolecular interactions.
Label-free detection technologies have been widely used to characterize biomolecular interactions without having to label the target molecules. These technologies exhi...
Cystatin C: a kidney function biomarker.
Renal function is essential for homeostasis. The kidneys play important pleiotropic roles including removal of metabolic waste products and maintenance of water-electr...
Electropolymerized phenol derivatives as permselective polymers for biosensor applications.
Amperometric biosensors are often coated with a polymeric permselective film to avoid electroactive interference by reducing agents present in the target medium. Pheny...
Quenching of the electrochemiluminescence of RU-complex tagged shared-stem hairpin probes by graphene oxide and its application to quantitative turn-on detection of DNA.
Efficient and stable quenching of electrochemiluminescence (ECL) of tris(2,2'-bipyridine)-ruthenium(II) (Ru(bpy)3(2+))/tri-n-propylamine (TPrA) system by graphene oxid...