Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor.
Cdc42 is a small guanosine triphosphatase (GTPase) that plays a central role in polarity development in diverse cell types. Since the activity of Cdc42 is dynamically ...
Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks.
In order to survive and function properly in the face of an ever changing environment, cells must be able to sense changes in their surroundings and respond accordingl...
Enzymes as Sensors.
Over the last few decades the development of new technologies, the fabrication of new materials, and the introduction of nanotechnologies created new trends in a serie...
Highly sensitive colorimetric immunosensor for influenza virus H5N1 based on enzyme-encapsulated liposome.
Development of simple but sensitive biosensor for influenza detection is highly important in immediate and effective clinical treatment. In this study, a sensitive col...
A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury.
Metallothioneins (MTs) are a family of cysteine-rich proteins whose biological roles include the regulation of essential metal ions and protection against the harmful ...
A novel versatile microbiosensor for local hydrogen detection by means of scanning photoelectrochemical microscopy.
The development of a versatile microbiosensor for hydrogen detection is reported. Carbon-based microelectrodes were modified with a [NiFe]-hydrogenase embedded in a vi...
Label-free electrochemiluminescent biosensor for rapid and sensitive detection of pseudomonas aeruginosa using phage as highly specific recognition agent.
A virulent phage named as PaP1 was isolated from hospital sewage based on a lambda phage isolation protocol. This phage showed a strong and highly specific binding abi...
Fabrication of sensitive enzymatic biosensor based on multi-layered reduced graphene oxide added PtAu nanoparticles-modified hybrid electrode.
A highly sensitive amperometric glucose sensor was developed by immobilization of glucose oxidase (GOx) onto multi-layer reduced graphene oxide (MRGO) sheets decorated...
Twin-layer biosensor for real-time monitoring of alkane metabolism.
Intracellular metabolic sensors can be used for efficient screening and optimization of microbial cell factories. In particular, the sensors are useful in acquiring in...
Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor.
This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold ...
Synthesis of novel nitrogen-doped carbon dots for highly selective detection of iron ion.
Herein, we report an eco-friendly and simple fluorescent nitrogen-doped carbon quantum dot (N-CQD) biosensor which was synthesized via a hydrothermal method using erha...
Illuminating Photochemistry of an Excitation Ratiometric Fluorescent Protein Calcium Biosensor.
Fluorescent protein (FP)-based biosensors have become an important and promising tool to track metal ion movement inside living systems. Their working principles after...
Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies.
Introducing sulfhydryl groups to biomolecules to functionalize gold nanorods (GNRs) is an attractive method that involves the creation of a strong Au-S bond. Previousl...
Dynamic Regulation of Auxin Response during Rice Development Revealed by Newly Established Hormone Biosensor Markers.
The hormone auxin is critical for many plant developmental processes. Unlike the model eudicot plant Arabidopsis (Arabidopsis thaliana), auxin distribution and signali...
Bioavailability of immobilized epidermal growth factor: Covalent versus noncovalent grafting.
In an effort to rationalize and optimize an antiapoptotic coating combining chondroitin sulfate (CS) and epidermal growth factor (EGF) for vascular applications, the a...
Direct detection of two different tumor-derived extracellular vesicles by SAM-AuNIs LSPR biosensor.
Extracellular vesicles (EVs) are abundant in various biological fluids including blood, saliva, urine, as well as extracellular milieu. Accumulating evidence has indic...
Bovine serum albumin as an effective sensitivity enhancer for peptide-based amperometric biosensor for ultrasensitive detection of prostate specific antigen.
Bovine serum albumin (BSA) was firstly implemented as an effective sensitivity enhancer for a peptide-based amperometric biosensor for the ultrasensitive detection of ...
FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells.
Förster Resonance Energy Transfer (FRET) microscopy is a useful tool in molecular biology and medical research to monitor and quantify real-time dynamics of protein-pr...
The universe of ANA testing: a case for point-of-care ANA testing.
Testing for total antinuclear antibodies (ANA) is a critical tool for diagnosis and management of autoimmune diseases at both the primary care and subspecialty setting...
Single Molecule Force Spectroscopy to Compare Natural versus Artificial Antibody-Antigen Interaction.
Biorecognition is central to various biological processes and finds numerous applications in virtually all areas of chemistry, biology, and medicine. Artificial antibo...