DNA Transformer for Visualizing Endogenous RNA Dynamics in Live Cells.
The functions of RNA are tightly regulated via diverse intracellular mechanisms. However, probing the complex dynamics of endogenous RNA in live cells is a challenging...
Label-free detection of exosomes using a surface plasmon resonance biosensor.
The development of a sensitive and specific detection platform for exosomes is highly desirable as they are believed to transmit vital tumour-specific information (mRN...
Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.
All biosensing platforms rest on two pillars: specific biochemical recognition of a particular analyte and transduction of that recognition into a readily detectable s...
Sandwich-structured nanoparticles-grafted functionalized graphene based 3D nanocomposites for high-performance biosensors to detect ascorbic acid biomolecule.
We present a highly sensitive and selective nano-biosensor for rapid, stable and highly reproducible detection of ascorbic acid (AA) in the presence of dopamine, uric ...
Ligand-induced conformational switch in an artificial bidomain protein scaffold.
Artificial proteins binding any predefined "target" protein can now be efficiently generated using combinatorial libraries based on robust protein scaffolds. αRep is s...
Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing.
In this work, three different aqueous solutions containing imidazole-based ILs with different alkyl chain lengths ([Cnmim]Br, n = 2, 6, 12) were adopted as the medium ...
Long-Range Surface Plasmon-Polariton Waveguide Biosensors for Human Cardiac Troponin I Detection.
Straight long-range surface plasmon-polariton (LRSPP) waveguides as biosensors for label-free detection are discussed. The sensors consist of 5-μm-wide 35-nm-thick gol...
Nanocrystalline diamond-based impedance sensors for real-time monitoring of adipose tissue-derived stem cells.
Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with bu...
A sensitive electrochemical genosensor for highly specific detection of thalassemia gene.
Beta thalassemias (βth) are the result of mutations in the β-globin gene. In this report, an electrochemical genosensor was made to detect the sequences anent with the...
3D biosensors in advanced medical diagnostics of high mortality diseases.
Cardiovascular diseases, cancer, and diabetes are high mortality diseases, which account for almost two thirds of all deaths worldwide. Their early detection and conti...
Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review.
Mucin 1 protein (MUC1) is a membrane-associated glycoprotein overexpressed in the majority of human malignancies and considered as a predominant protein biomarker in c...
Development of an advanced electrochemical biosensing platform for E. coli using hybrid metal-organic framework/polyaniline composite.
Because of numerous merits (e.g., the possibility of their synthesis in 1-D, 2-D, and 3-D forms, large surface-to-volume ratio, and flexible framework functionality), ...
A rapid method to detect and estimate the activity of the enzyme, alcohol oxidase by the use of two chemical complexes - acetylacetone (3,5-diacetyl-1,4-dihydrolutidine) and acetylacetanilide (3,5-di-N-phenylacetyl-1,4-dihydrolutidine).
A rapid and sensitive method has been devised in order to detect and estimate the synthesis of the enzyme alcohol oxidase (AOX) by fungi, by way of the use of two chem...
Analysis of Simple Half-Shared Transimpedance Amplifier for Picoampere Biosensor Measurements.
High-throughput recordings of small current are becoming more common in biosensor applications, including in vivo dopamine measurements, single-cell electrophysiology,...
Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification.
An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer's disease (AD)-related biomarker. Electroactive molybdophosphate ani...
Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors.
Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past ef...
Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.
The incorporation of AuNPs into polymerase chain reaction (PCR) has become a promising strategy to develop sensitive sensing platforms, due to desirable optical proper...
[Transition of the Field from Biochemical Engineering to Pharmaceutical Sciences during 40 Years of the Research].
This review reflects back over almost 40 years of the author's basic research conducted at Graduate School of Pharmaceutical Sciences, Osaka University, Japan. After p...
Amplification of Sensor Signals from Metal Mesh Device with Fine Periodic Structure.
Two types of metal mesh devices with hole diameters of 1.7 and 0.3 μm were prepared by an electroforming method. The metal mesh devices with hole diameters of 1.7 and ...
Technical note: Validation of an automated in-line milk progesterone analysis system to diagnose pregnancy in dairy cattle.
The in-line milk analysis system (IMAS) is an automated biosensor technology that samples and quantifies milk progesterone concentrations (P4c) at frequent intervals s...