Towards DNA methylation detection using biosensors.
DNA methylation, a stable and heritable covalent modification which mostly occurs in the context of a CpG dinucleotide, has great potential as a biomarker to detect di...
A sensitive electrochemical sensor for in vitro detection of parathyroid hormone based on a MoS
This paper reports a biosensor based on a MoS2-graphene (MG) composite that can measure the parathyroid hormone (PTH) concentration in serum samples from patients. The...
Functional mining of transporters using synthetic selections.
Only 25% of bacterial membrane transporters have functional annotation owing to the difficulty of experimental study and of accurate prediction of their function. Here...
Serum from obstructive sleep apnea patients induces inflammatory responses in coronary artery endothelial cells.
BACKGROUND AND AIMS: Obstructive sleep apnea (OSA) is characterized by intermittent airway obstruction and systemic hypoxia during sleep, which can contribute to an in...
Attomolar electrochemical detection of the BCR/ABL fusion gene based on an amplifying self-signal metal nanoparticle-conducting polymer hybrid composite.
In the last ten years, conjugated polymers started to be used in the immobilization of nucleic acids via non-covalent interactions. In the present study, we describe t...
A cell-based biosensor for nanomaterials cytotoxicity assessment in three dimensional cell culture.
Nanoparticles (NPs) are widely used in consumer and medicinal products. The high prevalence of nanoparticles in the environment raises concerns regarding their effects...
Determination of glucose in human stomach cancer cell extracts and single cells by capillary electrophoresis with a micro-biosensor.
Bioactive species in cells can provide information about signal transduction, cell function, and the effects of disease treatment. In this article, a novel micro-biose...
A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy.
Genetically encoded fluorescent protein (FP)-based biosensor probes are useful tools for monitoring cellular events in living cells and tissues. Because these probes w...
Design and Application of Multi-functional Electrogenerated Chemiluminescence Imaging Analyzer.
A multi-functional eletrogenerated chemiluminescence (ECL) imaging analyzer including both a photomultiplier tube and charged coupled device as detectors has been deve...
Fluorescence Resonance Energy Transfer-based Biosensor Composed of Nitrogen-doped Carbon Dots and Gold Nanoparticles for the Highly Sensitive Detection of Organophosphorus Pesticides.
The present article reports a novel biosensor for organophosphorus pesticides based on fluorescence resonance energy transfer (FRET) between nitrogen-doped carbon dots...
Scalable Production of Sensor Arrays Based on High-Mobility Hybrid Graphene Field Effect Transistors.
We have developed a scalable fabrication process for the production of DNA biosensors based on gold nanoparticle-decorated graphene field effect transistors (AuNP-Gr-F...
Old concepts, new molecules and current approaches applied to the bacterial nucleotide signalling field.
Signalling nucleotides are key molecules that help bacteria to rapidly coordinate cellular pathways and adapt to changes in their environment. During the past 10 years...
Characterization of Biosensors Based on Recombinant Glutamate Oxidase: Comparison of Crosslinking Agents in Terms of Enzyme Loading and Efficiency Parameters.
Amperometric l-glutamate (Glu) biosensors, based on both wild-type and a recombinant form of l-glutamate oxidase (GluOx), were designed and characterized in terms of e...
A MoS₂ Nanosheet-Based Fluorescence Biosensor for Simple and Quantitative Analysis of DNA Methylation.
MoS₂ nanomaterial has unique properties, including innate affinity with ss-DNA and quenching ability for fluorescence dyes. Here, we present the development of a simpl...
Homogeneous Electrochemical Biosensor for Melamine Based on DNA Triplex Structure and Exonuclease III-Assisted Recycling Amplification.
Abasic site (AP site) in the triplex structure can recognize specific target with high selectivity. In this study, this character was first applied to develop a simple...
A Nanofluidic Biosensor Based on Nanoreplica Molding Photonic Crystal.
A nanofluidic biosensor based on nanoreplica molding photonic crystal (PC) was proposed. UV epoxy PC was fabricated by nanoreplica molding on a master PC wafer. The na...
Direct and ultrasensitive optofluidic-based immunosensing assay of aflatoxin M1 in dairy products using organic solvent extraction.
Aflatoxin M1 (AFM1), a highly toxic secondary metabolite, is present in a wide range of dairy products. In this study, we designed a simple, low-cost, reusable, and ea...
An ancestral role for the mitochondrial pyruvate carrier in glucose-stimulated insulin secretion.
OBJECTIVE: Transport of pyruvate into the mitochondrial matrix by the Mitochondrial Pyruvate Carrier (MPC) is an important and rate-limiting step in its metabolism. In...
Portable microsystem integrates multifunctional dielectrophoresis manipulations and a surface stress biosensor to detect red blood cells for hemolytic anemia.
Hemolytic anemia intensity has been suggested as a vital factor for the growth of certain clinical complications of sickle cell disease. However, there is no effective...
High-efficient and high-content cytotoxic recording via dynamic and continuous cell-based impedance biosensor technology.
Cell-based bioassays were effective method to assess the compound toxicity by cell viability, and the traditional label-based methods missed much information of cell g...