Application of screen-printed microband biosensors to end-point measurements of glucose and cell numbers in HepG2 cell culture.
通过绝缘并切割丝网印刷的水基碳电极制备微带葡萄糖生物传感器,该电极含有酞菁钴(CoPC)氧化还原介体和葡萄糖氧化酶(GOD)。在37 °C静置条件下,于+0.4 V工作电位下,传感器在缓冲液中对葡萄糖产生安培响应,灵敏度为26.4 nA/mM,线性范围为0.45–9.0 mM;最佳pH为8.5,活化能计算为40.55 kJ/mol。...