Co-immobilized glucose oxidase and β-galactosidase on bovine serum albumin coated allyl glycidyl ether (AGE)-ethylene glycol dimethacrylate (EGDM) copolymer as a biosensor for lactose determination in milk.
本研究将牛血清白蛋白(BSA)在pH 8.0下吸附于25%交联密度的烯丙基缩水甘油醚–乙二醇二甲基丙烯酸酯共聚物微球(AGE-25)表面,形成含氨基、巯基和羧基的蛋白涂层。利用戊二醛、二琥珀酰亚胺基碳酸酯(DSC)和1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)分别活化BSA的NH2、SH和COOH基团,将黑曲霉葡萄糖氧化酶...